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Sir John Shakespeare II (c. 1530-September 1601) was an English nobleman, politician, and knight. He was Sir William Shakespeare's father and husband of Mary Arden.

Sir John Shakespeare II was the son of English nobleman Sir Richard Shakespeare I (1490-1561) and Abigail Webb (1493-1565), John II was named after his paternal grandfather the English politician Sir John Shakespeare I (1460-1521), John II great-grandfather was the English politician Sir Thomas Shakespeare (1430-1511).