The US FDA’s proposed rule on laboratory-developed tests: Impacts on clinical laboratory testing

PAFAH2
Identifiers
Aliases	PAFAH2, HSD-PLA2, platelet activating factor acetylhydrolase 2
External IDs	OMIM: 602344; MGI: 2140321; HomoloGene: 37309; GeneCards: PAFAH2; OMA:PAFAH2 - orthologs
Gene location (Human)
Chr.	Chromosome 1 (human)
End	25,998,117 bp
Gene location (Mouse)
Chr.	Chromosome 4 (mouse)
End	134,154,724 bp
RNA expression pattern
	Top expressed in
	Top expressed in
	More reference expression data
	; ;
	More reference expression data
Gene ontology
Molecular function
Cellular component
Biological process
	Sources:Amigo / QuickGO
Orthologs
	5051
	100163
	ENSG00000158006
	ENSMUSG00000037366
	Q99487; Q5SY00
	Q8VDG7
	NM_000437
	NM_001285872; NM_001285874; NM_001285875; NM_001285877; NM_133880
	NP_000428
	NP_001272801; NP_001272803; NP_001272804; NP_001272806; NP_598641
	Wikidata
View/Edit Human	View/Edit Mouse

Platelet-activating factor acetylhydrolase 2, cytoplasmic is an enzyme that in humans is encoded by the PAFAH2 gene.^[5]^[6]^[7] It is one of several PAF acetylhydrolases.

Function

This gene encodes platelet-activating factor acetylhydrolase isoform 2, a single-subunit intracellular enzyme that catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine). However, this lipase exhibits a broader substrate specificity than simply platelet activating factor. Two other isoforms of intracellular platelet-activating factor acetylhydrolase exist, and both are multi-subunit enzymes. Additionally, there is a single-subunit serum isoform of this enzyme.^[7]

References

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000158006 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000037366 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Hattori K, Adachi H, Matsuzawa A, Yamamoto K, Tsujimoto M, Aoki J, Hattori M, Arai H, Inoue K (Dec 1996). "cDNA cloning and expression of intracellular platelet-activating factor (PAF) acetylhydrolase II. Its homology with plasma PAF acetylhydrolase". The Journal of Biological Chemistry. 271 (51): 33032–8. doi:10.1074/jbc.271.51.33032. PMID 8955149.
^ Rice SQ, Southan C, Boyd HF, Terrett JA, MacPhee CH, Moores K, Gloger IS, Tew DG (Mar 1998). "Expression, purification and characterization of a human serine-dependent phospholipase A2 with high specificity for oxidized phospholipids and platelet activating factor". The Biochemical Journal. 330 ( Pt 3) (3): 1309–15. doi:10.1042/bj3301309. PMC 1219277. PMID 9494101.
^ ^a ^b "Entrez Gene: PAFAH2 platelet-activating factor acetylhydrolase 2, 40kDa".

Stafforini DM, McIntyre TM, Zimmerman GA, Prescott SM (Jul 1997). "Platelet-activating factor acetylhydrolases". The Journal of Biological Chemistry. 272 (29): 17895–8. doi:10.1074/jbc.272.29.17895. PMID 9218411.
Umemura K, Kato I, Hirashima Y, Ishii Y, Inoue T, Aoki J, Kono N, Oya T, Hayashi N, Hamada H, Endo S, Oda M, Arai H, Kinouchi H, Hiraga K (Mar 2007). "Neuroprotective role of transgenic PAF-acetylhydrolase II in mouse models of focal cerebral ischemia". Stroke: A Journal of Cerebral Circulation. 38 (3): 1063–8. doi:10.1161/01.STR.0000257981.09329.d2. PMID 17272759.
Schmidt EB, Koenig W, Khuseyinova N, Christensen JH (Jan 2008). "Lipoprotein-associated phospholipase A2 concentrations in plasma are associated with the extent of coronary artery disease and correlate to adipose tissue levels of marine n-3 fatty acids". Atherosclerosis. 196 (1): 420–4. doi:10.1016/j.atherosclerosis.2006.11.027. PMID 17157859.
Unno N, Sakaguchi T, Nakamura T, Yamamoto N, Sugatani J, Miwa M, Konno H (Jul 2006). "A single nucleotide polymorphism in the plasma PAF acetylhydrolase gene and risk of atherosclerosis in Japanese patients with peripheral artery occlusive disease". The Journal of Surgical Research. 134 (1): 36–43. doi:10.1016/j.jss.2006.02.058. PMID 16650870.
Marques M, Pei Y, Southall MD, Johnston JM, Arai H, Aoki J, Inoue T, Seltmann H, Zouboulis CC, Travers JB (Oct 2002). "Identification of platelet-activating factor acetylhydrolase II in human skin". The Journal of Investigative Dermatology. 119 (4): 913–9. doi:10.1046/j.1523-1747.2002.01859.x. PMID 12406338.
Min JH, Wilder C, Aoki J, Arai H, Inoue K, Paul L, Gelb MH (Apr 2001). "Platelet-activating factor acetylhydrolases: broad substrate specificity and lipoprotein binding does not modulate the catalytic properties of the plasma enzyme" (PDF). Biochemistry. 40 (15): 4539–49. doi:10.1021/bi002600g. PMID 11294621.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000158006 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000037366 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid8955149-5] Hattori K, Adachi H, Matsuzawa A, Yamamoto K, Tsujimoto M, Aoki J, Hattori M, Arai H, Inoue K (Dec 1996). "cDNA cloning and expression of intracellular platelet-activating factor (PAF) acetylhydrolase II. Its homology with plasma PAF acetylhydrolase". The Journal of Biological Chemistry. 271 (51): 33032–8. doi:10.1074/jbc.271.51.33032. PMID 8955149.

[pmid9494101-6] Rice SQ, Southan C, Boyd HF, Terrett JA, MacPhee CH, Moores K, Gloger IS, Tew DG (Mar 1998). "Expression, purification and characterization of a human serine-dependent phospholipase A2 with high specificity for oxidized phospholipids and platelet activating factor". The Biochemical Journal. 330 ( Pt 3) (3): 1309–15. doi:10.1042/bj3301309. PMC 1219277. PMID 9494101.

[entrez-7] "Entrez Gene: PAFAH2 platelet-activating factor acetylhydrolase 2, 40kDa".

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[2]

[3]

[4]

[5]

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The US FDA’s proposed rule on laboratory-developed tests: Impacts on clinical laboratory testing

Function

References

Further reading